Fig. 4

Pten over-expression in astrocyte-like glia is sufficient to extend lifespan without delaying development. a Drosophila glia comprise several distinct subsets, including the perineurial and subperineurial glia that form the blood-brain barrier, cortex glia that surround neuronal cell bodies, ensheathing glia that line the neuropil, and astrocyte-like glia whose processes infiltrate the neuropil. Panel adapted from [40]. b Survival curves show reduced lifespan for w^Dah;UAS-Pten/NP6293-GAL4;+ flies (red) compared to control w^Dah;NP6293-GAL4/+;+ (blue) and w^Dah;UAS-Pten/+;+ (black) flies. c Survival curves show reduced lifespan for w^Dah;UAS-Pten/NP2222-GAL4;+ flies (red) compared to control w^Dah;NP2222-GAL4/+;+ (blue) and w^Dah;UAS-Pten/+;+ (black) flies. d Survival curves show extended lifespan for w^Dah;UAS-Pten/+;alrm-GAL4/+ flies (red) compared to control w^Dah;+;alrm-GAL4/+ (blue) and w^Dah;UAS-Pten/+;+ (black) flies. For all lifespans, n > 140 deaths counted per group. e Egg-to-adult timing shows unaffected development timing for w^Dah;UAS-Pten/+;+ flies (black) compared to control wDah;+;+ flies (green). f Egg-to-adult timing shows delayed development timing for w^Dah;UAS-Pten/NP6293-GAL4;+ flies (red) compared to control w^Dah;NP6293-GAL4/+;+ flies (blue). g Egg-to-adult timing shows delayed development timing for w^Dah;UAS-Pten/NP2222-GAL4;+ flies (red) compared to control w^Dah;NP2222-GAL4/+;+ flies (blue). h Egg-to-adult timing shows unaffected development timing for w^Dah;UAS-Pten/+;alrm-GAL4/+ flies (red) compared to control w^Dah;+;alrm-GAL4/+ flies (blue). For all development assays, n > 70 flies counted per group. p values are from log-rank tests versus control group of that colour