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Fig. 1. | BMC Biology

Fig. 1.

From: Retargeting azithromycin analogues to have dual-modality antimalarial activity

Fig. 1.

Schematic of drug treatment regimens outlining the times of treatment and stage/time of parasitaemia measurement for assays used in this study. a Merozoite invasion of RBCs: Merozoites were drug treated prior to addition of RBCs. RBC invasion was measured at early ring stages (< 1 hr rings). b In-cycle: highly synchronous, early ring-stage parasites (0–4 hrs post-invasion) were treated with drug, with the resulting growth inhibition analysed at schizont stage (44 hrs post-invasion for P. falciparum and 26 hrs for P. knowlesi). c One cycle (0–72 hrs): highly synchronous, early ring-stage parasites (0–4 hrs post-invasion) were drug-treated and the resulting growth inhibition was measured after ~ 72 hrs of growth, post one cycle of re-invasion, at schizont stages. d 2 cycle (delayed death); highly synchronous, early ring-stage parasites (0–4 hrs post-invasion) were drug-treated and allowed to grow for 92 hrs before washing drug with fresh media (post second invasion cycle). Growth inhibition was assessed approximately 30 hrs later, at schizont stages (0–120 hrs post-invasion for P. falciparum and 0–92 hrs for P. knowlesi)

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