Haemoglobin fractionation of GSK-71, chloroquine and GSK-66-treated Plasmodium falciparum (3D7) parasites compared to an ethanol control. Scatter dot plots representing the relative levels of a haemoglobin, b free haem and c haemozoin in trophozoite-stage parasites following a 5 hr incubation with 10× IC50 (44 hr) concentration of GSK-71 (1100 nM), chloroquine (520 nM) and GSK-66 (70 nM) expressed as the fold change when compared to an EtOH control. Data is represented as the mean of > 3 paired replicates from three independent experiments with the error bars expressed as SEM. Significant differences were assessed using Student’s t test. Repeat measure data is available in Additional file 15 Supporting Value Data. d A panel of representative Giemsa-stained parasites treated with 10× IC50 (44 hr) concentration of GSK-71, chloroquine, GSK-66 and the ethanol negative control after 5 hrs.