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Fig. 3 | BMC Biology

Fig. 3

From: Spatial and temporal modulation of enterotoxigenic E. coli H10407 pathogenesis and interplay with microbiota in human gut models

Fig. 3

Dynamics of ETEC membrane integrity determined by PMA-qPCR in the TIM-1 and M-SHIME. ETEC membrane integrity was estimated by PMA-qPCR (Enterobacteriaceae 16S rRNA and ETEC gspD genes for TIM-1 and M-SHIME samples, respectively) and expressed as the average percentage of intact (green) and damaged (shade of pink) ETEC cells. Damaged ETEC cells were obtained after deducting the number of intact cells from the total ETEC cells measured by qPCR. ETEC membrane integrity was determined over time: a in the inoculum, gastric and ileal effluents of the TIM-1 (n = 4) and b in the inoculum, ileum, and ascending colon of the M-SHIME (n = 6). When inter-individual variability was important, a second doughnut was represented

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