Fig. 4

Dynamics of ETEC virulence gene expression in different gastrointestinal regions of the a TIM-1 and b M-SHIME. Results were determined by RT-qPCR, expressed and colored according to the log₂ fold-change. For reasonable statements, statistically significant differential expression had to meet two criteria: a mean log₂ fold change expression of ≥ 1 (induction denoted in shade of green) or ≤ − 1 (repression denoted in shade of red) and a p ≤ 0.05. The statistically significant differences in average of log₂ gene expression at each time points for the four (TIM-1) or six (M-SHIME) replicates were compared to the initial inoculum (T0) and marked by the white frames in the heatmap, as determined by the Friedman post hoc Nemenyi test