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Fig. 4 | BMC Biology

Fig. 4

From: Deep learning-enabled analysis reveals distinct neuronal phenotypes induced by aging and cold-shock

Fig. 4

PVD neuronal structure undergoes morphological changes upon exposure to acute cold-shock. a Schematic of acute cold-shock assay. Nematodes were cultured at 20 °C until day 2 of adulthood, split into four plates, and cold-shocked for 4, 8, 16, or 24 h. Fluorescence microscopy was conducted before exposure to cold-shock and after specific periods of shock. bd Average number of beads, average of mean bead size, and average inter-bead distance of anterior and posterior regions of PVD as nematodes experienced various duration of cold-shock. The lines are the 25th percentile, mean, and 75th percentile. Whisker is the standard deviation. Statistical analysis was performed with one-way ANOVA followed by Tukey’s (b, c) or Steel-Dwass (d) tests for multiple comparison with equal or unequal variance assumptions, respectively, and significance level determined using Bonferroni correction (α = 0.016) for multiple feature comparisons. *P < 0.016, **P < 0.001, and ***P < 0.0001. e Illustration of distinct beading patterns in aging and acute cold-shock based on the inter-bead distance. Inter-bead distance decreases with aging while it increases with cold-shock. Error bar is SEM

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