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Fig. 5 | BMC Biology

Fig. 5

From: Deep learning-enabled analysis reveals distinct neuronal phenotypes induced by aging and cold-shock

Fig. 5

PVD morphological variation phenotypes caused by acute cold-shock are reversible. a Schematic of post-cold-shock rehabilitation treatment assay. Nematodes were cultured at 20 °C until day 2 of adulthood and were exposed to cold-shock for 16 h. To perform recovery, the population was split into three plates at either 15 °C, 20 °C, or 25 °C for 1 day. bd Average number of beads, average of mean bead size, and average inter-bead distance of PVD neuron as nematodes experienced cold-shock for 16 h and undergo rehabilitation at 3 different temperatures. The lines are the 25th percentile, mean, and 75th percentile. Whisker is the standard deviation. Statistical analysis was performed with one-way ANOVA followed by Tukey’s (c, d) or Steel-Dwass (b) tests for multiple comparison with equal or unequal variance assumptions, respectively, and significance level determined using Bonferroni correction (α = 0.016) for multiple feature comparisons. *P < 0.016, **P < 0.001, and ***P < 0.0001. Error bar is SEM

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