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Fig. 6 | BMC Biology

Fig. 6

From: Genome-enabled insights into the biology of thrips as crop pests

Fig. 6

Identification of co-expressed genes (modules) and gene ontologies associated with three developmental stages of Frankliniella occidentalis. a Association between modules of co-expressed genes (colored boxes stacked on left of figure) and developmental stage, depicting the gene correlation network. Weighted gene co-expression network analysis [43] was performed on a matrix of normalized read counts (FPKM values) obtained from a published F. occidentalis RNA-seq study involving three biological replicates of healthy first-instar larvae, propupae, and adults (mixed males and females) [24]. Modules of co-expressed genes were determined by the dynamic tree cutting algorithm with a minimum of 20 genes per module. Modules that exhibited the highest correlation (red color) with a developmental stage are indicated by an asterisk (*). Transcript IDs of co-expressed genes within these significant stage-associated modules are presented in Additional file 3. b–d REVIGO (REduce and Visualize Gene Ontologies, [255]) was used to visualize specific GO terms comprised of non-redundant sequences enriched in each developmental stage; sizes of delineated blocks indicate the number of genes within each GO category. Refer to Additional file 3 for more detailed REVIGO maps with identities of each GO term (block) indicated

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