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Fig. 2 | BMC Biology

Fig. 2

From: ACBE, a new base editor for simultaneous C-to-T and A-to-G substitutions in mammalian systems

Fig. 2

Base editing of ACBE in endogenous loci of HEK293 cells. a Summary of base editing type with ACBE at 45 sgRNAs targeting the human endogenous loci. b Average C-to-T and A-to-G base editing spectrum of ACBE for endogenous targeting sites in HEK293 cells based on the statistical analysis of the 45 sgRNAs. The values indicated the mean, and the untreated samples served as control. c The representative Sanger sequencing results of the cell samples transfected with different base editors showed the target base substitutions in the targeting sites P53-G7, P53-G8, LMNA-G1, LDHA-G1, and PGK1-G4. The C-to-T and A-to-G substitutions were marked with red and black arrows, respectively. Three independent experiments were performed. d The editing effects on the target sites P53-G7, P53-G8, LMNA-G1, LDHA-G1, and PGK1-G4 were generated by ACBE and CBE + ABE, respectively. Different parts with various colors in the pie chart showed the frequencies of the corresponding mutation type. The result was based on the representative amplicon deep sequencing (n = 3)

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