Fig. 3From: ACBE, a new base editor for simultaneous C-to-T and A-to-G substitutions in mammalian systemsACBE-mediated dual-function base editing in MEFs and PFFs. a The base editing effects of ACBE in mstn and tyr loci of MEFs; b The base editing effects of ACBE in TYR, FANCA, and LMNA loci of PFFs. The efficiencies of base editing (a, b) were quantified from amplicon deep sequencing. Values and error bars indicate mean ± s.e.m. of three independent experiments. An untreated cell sample served as control. c, d The efficiencies of ACBE-mediated simultaneous C-to-T and A-to-G base substitutions in the targeting loci of MEFs (c) and PFFs (d) were calculated the reads of amplicon deep sequencing. N.S., not significant (P > 0.05); *(0.05 > P ≥ 0.001); **(0.01 > P ≥ 0.0001); ***(P < 0.0001) (two-tailed t test)Back to article page