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Fig. 4 | BMC Biology

Fig. 4

From: ACBE, a new base editor for simultaneous C-to-T and A-to-G substitutions in mammalian systems

Fig. 4

ACBE-mediated base editing effects with different spacer lengths. a Schematic design for different spacer length of EGFP-Stop2. The red sequence indicated the PAM. b Flow cytometry results for testing different spacer lengths of EGFP-Stop2, n = 3. Untreated cell samples served as control. c, d The sequences represented the serial sgRNAs with different spacer lengths at P53-G8 (c) and P53-G9 (d) loci. The red sequence indicated the PAM. e, f Summary of ACBE-mediated base editing patterns and efficiencies of all C and A in P53-G8 (e) and P53-G9 (f) sites using sgRNAs with different spacer lengths. All values and error bars above indicate mean ± s.e.m. of three independent experiments. The controls were untreated cell samples. g, h The frequencies of simultaneous A > G and C > T mutation for P53-G8 (g) and P53-G9 (h) loci with different spacer length gRNA

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