Fig. 3

Probing axon degeneration kinetics in vivo. a Schematic illustrating single and double lesion experiments from different regions of the axon. b Average fragmentation onset time for double lesioned distal axons (black circles, n = 13 axons, 10 mice), double lesioned proximal axons (open circles, n = 8 axons, 7 mice) and single lesioned axons (blue circles, n = 18 axons, 9 mice). Grey lines link data points from double lesioned distal and proximal segments along the same axon (n = 5 axons, 3 mice). Wilcoxon test, *** p < 0.001. c Slower fragmentation rate of shorter axons undergoing roWD (blue points) compared to longer axons undergoing WD (grey points) after a single axonal lesion, **** p < 0.0001. d No correlation between roWD fragmentation rate and axon length (blue points, n = 21 axons, 13 mice, p > 0.05) or for longer axon WD fragmentation rate and axon length (e, grey points, n = 14 axons, 14 mice, p > 0.05)