Fig. 5.

Mean ΔΔG (in kcal/mol) and fitness of amino acid mutations caused by the substitution of each of the four nucleobases. a Computed 〈ΔΔG〉 of SBSs in the \({\mathcal {M}_{\text {PoP}}}\) set. b Computed 〈ΔΔG〉 of SBSs as a function of the residue solvent accessibility (RSA) in the \(\mathcal {M}\)PoP set. c Fitness score of mutations measured via the minimum inhibitory concentration (MIC) to β-lactam amoxicillin [57]. d Computed 〈ΔΔG〉 of SBSs as a function of the RSA in the \(\mathcal {M}_{\text {Exp}}\) set. Note that here only three RSA bin (0–20%, 20–50%, 50–100%) are considered due to the limited number of entries