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Fig. 3 | BMC Biology

Fig. 3

From: Natural food intake patterns have little synchronizing effect on peripheral circadian clocks

Fig. 3

In vivo imaging and peripheral tissue phases. Every 4 h, mice were imaged after being lightly anesthetized and injected s.c. with luciferin. a Ventral images from a representative mouse: note that peak PER2::LUC bioluminescence in the SMG occurs at ZT21 in all conditions, whereas peak bioluminescence in the liver occurs earlier only in the TRF light-fed condition. b Mean bioluminescence is plotted as a function of time of day (n = 9). Statistical results from a 2-way repeated measures ANOVA are shown (main and interaction effects of zeitgeber time and food condition). A significant interaction suggests a change in the phase or amplitude of the rhythm. c The phase of each tissue was calculated from the direction of the mean resultant vector and plotted (± SE). p value indicates the result of a 1-way repeated measures ANOVA (feeding condition as the independent variable). Conditions that do not share a letter are significantly different (Tukey test, p < .05). Individuals are shown by gray traces

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