Fig. 2

Decreased proliferation of neural progenitors in the dentate gyrus (DG). NSPA-KO mice. a Representative immunostaining of DCX in the DG of WT and NSPA-KO mice. Scale bar: 100 μm. b Representative immunostaining of Ki67 and the mature neuronal marker NeuN in the DG of WT and NSPA-KO mice. Scale bar: 100 μm. Quantification of total number of Ki67+ cells in the SGZ. (mean ± SEM; n = 10 mice per condition; ***P < 0.001, t-test). c Schematic representation of the experimental procedure. Animals received a daily dose of 100 mg kg⁻¹ BrdU for 3 days and were sacrificed 2 weeks later. Immunostaining of BrdU in the DG of WT and NSPA-KO mice. Nuclei were stained with NucBlue (Nuc). Scale bar: 50 μm. Quantification of total number of BrdU+ cells in the granule cell layer (GCL) of the DG (mean ± SEM; n = 4 mice per condition; *P < 0.05, Mann-Whitney test). d Representative immunostaining of BrdU and DCX. Scale bar: 20 μm. Quantification of the percentage of BrdU-positive cells expressing DCX (mean ± SEM; n = 4 mice per condition; n.s, non-statistical differences, Mann-Whitney test). Source data values are included in Additional file 2