Fig. 8

Bone derived choline regulates insulin sensitivity. a Schematic diagram outlining the mechanisms by which ceramide and choline and linked. b Mouse serum ceramide and choline (c) analysis by LC-MS/MS (ceramide) and assay (choline). d GTT. e ITT. f Dissected fat depot weights. g Quantification of epididymal fat adipocyte diameter and representative histology. Data are represented as mean ± S.E.M (c–g) n = 3–5 per group. Different letters above the error bar show significant difference at p < 0.05 and the data were assessed as follows: c, f, g Two-way ANOVA with multiple comparisons. b One-way ANOVA with Dunnet’s or Tukey’s tests for multiple comparisons. d, e repeated measures two-way ANOVA with Sidak’s multiple comparisons test