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Fig. 2 | BMC Biology

Fig. 2

From: Bioengineered human skeletal muscle capable of functional regeneration

Fig. 2

Media supplementation of remixed engineered muscles increases morphological maturity and increases functional capacity. a Representative micrographs stained for myosin heavy chain (MyHC) – green and Nuclei (DAPI) – blue. Scale bar 100 μm. b–f Graphs displaying; percentage MyHC coverage, myotubes per mm2, myotube cross-sectional area (CSA), nuclei per section and normalised force (normalised to control), respectively. Mean tetanus force at control 45.9μN, 0.96 kPa; twitch force 22.7μN, 0.048 kPa. All graphs display mean ± S. D. g, h Experimental schemes showing conditions coloured similarly to graphs. Scheme (g) applies to a–f whilst scheme (h) applies to i–n. i Representative micrographs stained for myosin heavy chain (MyHC) – green and Nuclei (DAPI) – blue. Scale bar 100 μm. j Normalised force (normalised to -FGF condition) mean tetanic force for -FGF condition 14.3μN, 0.10 kPa; twitch 6.2μN, 0.35 kPa. k–n Graphs displaying; Percentage MyHC coverage, myotubes per mm2, myotube cross-sectional area (CSA), nuclei per section respectively. All graphs display mean ± S. D, individual repeat means are displayed as dots. Statistical significance from control (a–f) and -FGF (i–n) is denoted as *p ≤ 0.05, ***p ≤ 0.001, n = 9 samples across 3 repeats

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