Fig. 4
From: Bioengineered human skeletal muscle capable of functional regeneration
Human engineered muscles regenerate functionally and morphologically following injury. a Representative micrographs of engineered muscle cross-sections. Stained for MyHC (green) and Nuclei (DAPI, blue). Scale bar represents 100 μm. b Representative force traces used for tetanus and twitch force measurements. c Normalised force measurements across recovery. Means at control; tetanus – 101.6 μN, 0.19 kPa; twitch – 36.6μN, 0.072 kPa. d Normalised morphological measures across recovery. Means at control; percentage MyHC coverage – 13.0%, myotube cross-sectional area (CSA) – 132.6 μm2, myotubes per mm2 –971.6. b–d All graphs display control normalised means ± S. D, individual repeat means are displayed as points. Dashed line represents level at control, normalised to 1, on all graphs. Statistical significance from control is denoted as *p ≤ 0.05, **p ≤ 0.01 and ***p ≤ 0.001, n = 15 samples across 5 repeats