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Fig. 2 | BMC Biology

Fig. 2

From: The CBP KIX domain regulates long-term memory and circadian activity

Fig. 2

Differential gene expression analysis in the dorsal hippocampus of CBPKIX/KIX mice following spatial learning. a Experimental scheme: Total RNA was extracted from the dorsal hippocampus of CBPKIX/KIX and control littermates 1 h after MWM training (day 3, trial 4) and 1 h after contextual fear conditioning (n = 7 CBPKIX/KIX, n = 7 controls). Each set of experiments was sequenced separately but analyzed together to identify common genes responsive to hippocampus-dependent learning. b Volcano plot illustrating differentially expressed genes between CBPKIX/KIX and control mice after learning (common between MWM and contextual fear conditioning). c KEGG pathway enrichment network analysis showing significant KEGG pathways enriched in the downregulated genes. Top significant pathways are shown in the bar graph. Mammalian circadian rhythm pathway (p = 1.55 × 10−5). d Heat map of IPA upstream regulator analysis on differentially expressed genes in CBPKIX/KIX mice following training. Most significant regulators are on the top of the heat map. CREB1, also known as CREB, is the top-predicted upstream regulator of differentially expressed genes (FDR < 0.05) in the CBPKIX/KIX mice compared to wildtype littermates (predicted inhibition, z-score = − 3.349, p = 1.49 × 10−14. e, f Known interactions of differentially expressed genes (FDR < 0.05) in CBPKIX/KIX vs control following learning predicting CREB1 (e) and CLOCK (f) as upstream regulators

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