Fig. 1
From: The selectivity filter of the mitochondrial protein import machinery
Fluorescence microscopy of yeast cells expressing EGFP-labeled segments of AAC. a Upper panel: Images of yeast cells expressing wild type AAC (AACWT, ADP/ATP carrier of Neurospora crassa) containing an EGFP moiety at the C-terminus. Second panel: Cells expressing AACΔpos (all positively charged residues of the matrix loops exchanged against glycine). Third panel: AAC module III (residues 221–313). Lower panel: AAC module IIIΔpos (the positively charged residues of the matrix loop exchanged against glycine). MitoTracker Orange was used for labelling of the mitochondria. Scale bars, 2 μm. b Yeast cells expressing EGFP-labeled segments of AAC module III. c Amino acid sequence of Neurospora crassa AAC residues 238–305. The assignments are based on the crystal structure of bovine ADP/ATP carrier [1, 40]. Red, positively charged residues; blue, negatively charged residues