Fig. 5

Binding of C-terminal segments of AAC to isolated mitochondria. [³⁵S]-labeled segments of the AAC fused to DHFR were synthesized in reticulocyte lysate and incubated with isolated yeast mitochondria. The samples were ATP-depleted by incubation with apyrase. The mitochondria were reisolated, and the relative amounts of radiolabeled polypeptides were determined. The amounts of radiolabeled protein bound to untreated mitochondria were compared to the average amounts of the same protein bound to trypsin-pretreated mitochondria. SD, n = 3. TMD, transmembrane domain; ML3, matrix loop of module III