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Table 1 S. cerevisiae strains used in this study

From: The selectivity filter of the mitochondrial protein import machinery

Yeast strains

Source

Acc. No.

BY4742 (WT) MATα his3Δ1 leu2Δ0 lys2Δ0 ura3Δ0

[101]

Y10000

BY4742 tom70::kanMX4

EUROSCARF

Y17244

BY4742 pex19::kanMX4

EUROSCARF

Y13762

K39 (CEN.PK 111-61A) MATα ura3–52 leu2–3.1/2 his3Δ1 MAL2-8c MAL3 SUC3 GAL

Prof. Heinisch, Osnabrück (D)

 

K39 pUG35-AAC (Neurospora crassa)

This study

 

K39 pUG36-AAC∆pos

This study

 

K39 pUG35-AAC(221–313)

This study

 

K39 pUG35-AAC∆pos(221–313)

This study

 

K39 pUG36-AAC(238–313)

This study

 

K39 pUG36-AAC(278–313)

This study

 

K39 pUG36-AAC(238–277)

This study

 

K39 pUG36-AAC(238–305)

This study

 

K39 pUG35-AAC(221–277)

This study

 

K39 pUG35-AAC(278–296 + 238–277)

This study

 

K39 pUG36-AAC(238–277 + 221–237)

This study

 

K39 pUG36-AAC(238–313)R290Q

This study

 

K39 pUG36-AAC(238–300)

This study

 

K39 pUG36-AAC(238–290)

This study

 

K39 pUG36-AAC(246–313)

This study

 

K39 pUG36-AAC(258–313)

This study

 

K39 pUG36-AAC(33–97)

This study

 

K39 pUG36-AAC(138–201)

This study

 

K39 pUG36-DIC(227–298) (Saccharomyces cerevisiae)

This study

 

BY4742 tom70::kanMX4 pUG35-AAC

This study

 

BY4742 tom70::kanMX4 pUG36-AAC(238–305)

This study

 

BY4742 tom70::kanMX4 pPC97-Tom70WT

This study

 

BY4742 tom70::kanMX4 pPC97-Tom70C141A

This study

 

BY4742 tom70::kanMX4 pPC97-Tom70C439A

This study

 

BY4742 tom70::kanMX4 pPC97-Tom70C450A

This study

 

BY4742 tom70::kanMX4 pPC97-Tom70E473,542,577Q

This study

 

BY4742 pUG35-Ugo1 (Saccharomyces cerevisiae)

This study

 

BY4742 tom70::kanMX4 pUG35-Ugo1

This study

 

BY4742 pUG35-Ugo1(150–290)

This study

 

BY4742 tom70::kanMX4 pUG35-Ugo1(420–491)

This study

 

BY4742 (tom70∆/tom71∆) tom70::kanMX4 tom71::natMX6

This study

 

BY4742 tom70∆/tom71∆ pUG35-AAC (Neurospora crassa)

This study

 

BY4742 tom70∆/tom71∆ pUG36-AAC(238–305)

This study

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