Fig. 3

Accumulation of deletions induced by Polg^D181A expression are brain region-specific and ageing-dependent. a Dot plot illustrating the age-dependent increase in the load of SNVs in Polg^D181A mice across the investigated brain regions (as indicated by the colour legend) normalised to the mean of WT samples at 10 weeks. Grey diamonds indicate the mean of WT-derived brain region samples for reference (same as in Fig. 1f). Red diamonds indicate the mean of Polg^D181A-derived brain region samples and the 95% confidence interval is shown. Three-way ANOVA (age, region, and animal) of Polg^D181A-derived samples showed that age significantly contributed to deletion levels (p values of post hoc Tukey’s test are shown). p values of three-way ANOVA (age, genotype, region) with post hoc Tukey’s test are shown for each age group. For region contribution, we found a significant contribution of COR, NAc, and PVT to deletion levels in Polg^D181A mice using a linear model for main effects. b Chord diagrams indicating the deletions accumulated at 10, 50, and 80 weeks in DR and PVT from WT and Polg^D181A mice. Data is normalised pr. brain region, and the width of each gene indicates the summed allele fraction of deletions spanning the indicated gene(s). The colour of the chord indicates the gene in which the breakpoint 5′ position is located. Plots were made using circlize