Fig. 3

Phylogenetic analyses of SOD1 or SOD1-like proteins from nematodes and mammals, and neighboring and conserved intra- and extracellular SOD isoforms in filariae. a Phylogeny of the SOD1 group in nematodes, human, and mouse. The predicted signal peptide of SOD1-like in filariae is indicated with a circled 'SP' and experimental evidence from the literature is indicated by a star. The red branch indicates SOD1-like in filaria, and the pink branch indicates SOD1 in filaria. See Additional file 1: Fig. S5 for a more detailed phylogeny without compressed nodes. The assigned shape and color indicate clades and subclades, respectively. b The branch length of SOD1 and SOD1-like in filaria (pink and red branches in panel a, respectively). Each genus contains multiple species, and the branch length of a gene in each species is added as their last common node. P values were calculated using Student’s t test. c Neighboring in position and high amino acid identity between Sod1 and Sod1-like in the filariae. AA represents amino acids, and Ks is the synonymous mutation rate. A line connecting two genes indicated that they were neighboring in position. d Conserved gene loci around Sod1 and Sod1-like in Filarioidea with gene number more than six in the scaffold. e Conservation of Sod1 and Sod1-like in structure and genomic sequences. Genomic identity was calculated at a 10-bp sliding window based on pairwise alignment