Fig. 1

Resilin localises to distinct regions of the fly body. a Pro-Resilin-GFP (red) is detected in the head region of the pupa. b In the adult fly, spots are found at the leg joints, at the wing articulation and on the flanks of the abdomen. c In the head, Pro-Resilin-GFP marks large patches around the cibarium and the labellum. d, e By higher magnification, several small Pro-Resilin-GFP patches are detected along the length of the leg, close to the joints and at the wing articulations. Details of the Pro-Resilin-GFP signal in the leg are shown in Additional file 3: Figure S3. f Small spots are visible at the tracheal endings. g Pro-Resilin-GFP is also detected at the hair bases. The GFP signal was merged with the bright-field image in a–g. Images were recorded on a Leica DMi8 microscope. Details of the microscope settings are described in the “Methods” section. Labelling: br, bristle; ci, cibarium; co, coxa; fr, femur; lb, labellum; sp, spiracle; tb, tibia; th, trochanter; tr, tarsus; txs, thorax, exact location of signal unclear; wa, wing articulation