Fig. 2
From: Resilin matrix distribution, variability and function in Drosophila
Pro-Resilin-GFP is expressed in the proboscis. A, A′ The labellum and the cibarium (http://flybase.org/cgi-bin/cvreport.pl?id=FBbt:00004526) contain large amounts of Pro-Resilin-GFP. A, A″ These areas are marked by DT as in non-transgenic wild-type flies (B). C, D In pro-resilinRNAi and pro-resilincc-ATG flies the DT signal persists. Reduction of the DT signal is detectable by the Fiji software. Occasionally, the pseudotrachea (pt) (http://flybase.org/reports/FBim0000834) do not contain Pro-Resilin-GFP, but display a weak auto-fluorescence. We consider this as background signal. The asterisk (*) marks auto-fluorescence of internal tissues after dissection. Images were generated with a Zeiss LSM880 confocal microscope. The excitation (ex) and emission (em) wavelengths are indicated in the images. Those shown in A–A‴ were obtained by the normal confocal mode, while those shown in B–D were produced by the fast airyscan mode. Details of the respective settings are described in the “Methods” section. Labelling: ci, cibarium; lb, labellum; pt, pseudotrachea