Fig. 3

In legs, some Pro-Resilin-GFP signal does not coincide with a DT signal. A–A″ At the femur-to-tibia transition, we detect a Pro-Resilin-GFP patch, that does not coincide with a DT signal. B–B″ In the joint between the tibia and the tarsus, there is a star-like area of Pro-Resilin-GFP signal. This patch is not covered completely with a DT signal. C–C″ The tarsi show an array of Pro-Resilin-GFP signal. The DT signal does not overlap perfectly with the Pro-Resilin-GFP signal. The asterisk (*) marks auto-fluorescence of internal tissues after dissection. A Leica DMi8 microscope was used for imaging. The excitation (ex) and emission (em) wavelengths are indicated in the images. Details of the microscope settings are described in the “Methods” section. Labelling: fr, femur; th, trochanter; tb, tibia; tr, tarsus; txs, thorax, exact location of signal unclear