Fig. 4
From: Resilin matrix distribution, variability and function in Drosophila
Several patches of Pro-Resilin-GFP signal characterise the wing articulation. As shown in Fig. 1, Pro-Resilin-GFP marks five patches in the wing articulation. A–A″ By confocal microscopy, occasionally, one or two of these patches are out of focus and are therefore not visible. It is, however, evident that the DT signal intensity varies independently of the signal intensity of GFP. B The DT signals are also present in wild-type non-transgenic flies. C DT signal intensity is obviously reduced in pro-resilinRNAi flies. Patches are numbered in each image. The asterisk (*) marks auto-fluorescence of internal tissues after dissection. Images were generated on a Zeiss LSM880 confocal microscope. The excitation (ex) and emission (em) wavelengths are indicated in the images. They were obtained by the normal confocal mode. Details of the respective settings are described in the “Methods” section