Fig. 6

DT signal intensities are reduced in pro-resilin^RNAi flies. We measured and compared the DT signal intensities in wild-type and pro-resilin^RNAi wing articulations and trochanter following two statistical calculations. First, intensities were simply averaged and compared. In the second approach, we chose the intensity of 4 × 10⁶ counts/pixel as a threshold value to test how many samples passed this threshold (see the “Methods” section). a The areas of the wing articulations and the trochanter have similar sizes in wild-type and pro-resilin^RNAi flies. b In one approach, the DT intensities in both the wing articulations and the trochanter were significantly reduced in pro-resilin^RNAi flies compared to wild-type flies. c According to the other approach, 100% of wild-type wing articulations and trochanter had at least an intensity value of 4 × 10⁶ counts/pixel, while only about 13% and 38% of pro-resilin^RNAi wing articulation and trochanter samples, respectively, reached this value. This method also revealed that at least in the wing articulations, the DT signal accumulates during ageing. This accumulation is attenuated in pro-resilin^RNAi wing articulations. The sample sizes (n) were as follows: wild-type legs 64, pro-resilin^RNAi legs 62, wild-type (old and young) and pro-resilin^RNAi wing articulations 35