Fig. 5.
Unique spectral classification with Tbar. a Average ΔF/F0 traces (blue line, mean; light blue area, standard deviation) for all neurons identified in seven 5-dpf larvae with the given Tbar (indicated in each panel). Number of neurons: 2243 (Tbar 1), 581 (Tbar 2), 49 (Tbar 3), 51 (Tbar 4), 4509 (Tbar 8), 308 (Tbar 9), 88 (Tbar 10), and 14 (Tbar 11). The experimental trace of each neuron was first averaged over the three repetitions of the L1/L2/L3/L4 stimuli before averaging among different neurons. Standard deviations were calculated over all data (made of the three repetitions for each neuron, over all neurons found in each class for the seven larvae tested). Colored tick marks above each graph show the timing of the different stimuli. Only the classes with a total numerical consistency above 10 neurons are shown in the figure. b Anatomical distributions of neurons for each Tbar class. Anatomical areas are classified and indicated as in the rest of the paper: Telencephalon (T), eye (E), diencephalon (D), mesencephalon (M), rhombencephalon (R), and spinal cord (S). Values shown are averages and stderr calculated over the seven normalized distributions measured with the 7 larvae for each Tbar. The bars for the last plot (Tbar 11) do not add to 1 because the distributions were normalized to 1 for each larva across the anatomical areas, but not all larvae tested actually contributed cells to this Tbar class (i.e., some larvae contributed 0 into averaging). c Anatomical localization of some example neurons for each of the Tbar classes shown in the previous panels. Each neuron is shown as a dot (not drawn to scale, for visibility) and colored according to its responses with the color coding used throughout the paper. The gray border around neuron #12 indicates a negative T value. Scale bar, 150 μm. d Experimental ΔF/F0 traces for the neurons shown in c. The scales were chosen to optimize visibility in each graph and are not shown for compactness of the figure; the two ticks shown on the vertical axis are as follows for each panel starting from top left: (− 0.5, 2.2), (− 0.3, 1.2), (− 0.3, 1.4), (− 0.6, 4.3), (− 0.5, 3.7), (− 0.3, 1.4), (− 0.6, 2.1), (− 0.5, 3.0), (− 0.3, 1.3), (− 0.4, 3.4), (− 0.5, 4.0), and (− 0.4, 0.2)