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Fig. 5 | BMC Biology

Fig. 5

From: Genome-scale CRISPR screening at high sensitivity with an empirically designed sgRNA library

Fig. 5

CRISPR screens conducted at a high dynamic range predict the cutting efficiency of sgRNAs based on mild viability phenotypes. a A mixture model was used to divide control sgRNAs of the HD CRISPR library A in the single cell clone SCC12 screen into three groups: (1) sgRNAs with a target-dependent viability phenotype (red), (2) sgRNAs with a small target-independent phenotype likely caused by a double-strand break (blue), and (3) sgRNAs with no phenotype due to a lack of DNA cutting (yellow). Log2 fold change distributions of targeting and non-targeting control sgRNAs are indicated as dashed and solid curves, respectively. b Number of sgRNAs targeting nonessential HAP1 genes associated with each phenotype group. Nonessential genes were determined using MAGeCK which requires no prior knowledge for analysis. sgRNAs are stratified based on their design: “empirical essential” sgRNAs target context-specific essential genes and were selected for the HD CRISPR library based on their previous on-target phenotypes. “Empirical nonessential” sgRNAs are part of previously published libraries and target broadly nonessential genes. They were selected based on their lack of outlier phenotypes. De novo sgRNAs were designed using the software cld [34]

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