Fig. 6
From: Genome-scale CRISPR screening at high sensitivity with an empirically designed sgRNA library
HAP1 cells are highly dependent on Yamanaka factors and the Fanconi anemia pathway. a HAP1 context-dependent essential genes are enriched for genes comprising the Fanconi anemia pathway as well as factors known to induce pluripotency. The y-axis provides an estimate of the percentage of essentiality in other cell lines previously screened. Fanconi anemia pathway genes are highlighted in red, Yamanaka factors are shown in blue. HAP1-specific nonessential genes are highlighted in green. nt ctrl = non-targeting control. b Crystal violet staining of HAP1 cells treated with various siRNAs targeting SOX2, POU5F1, and KLF4 and a non-targeting control. Shown is a representative result of three independent experiments. c Essentiality of known cancer drivers, Fanconi anemia pathway genes, and pluripotency factors across cell lines representing different cancer cell types in comparison to HAP1 cells and human embryonic stem cells