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Fig. 1. | BMC Biology

Fig. 1.

From: Competition for nutritional resources masks the true frequency of bacterial mutants

Fig. 1.

a Daily accumulation and distribution of PCMs in 17 independent cultures. 109 MG1655 bacteria were plated on each TG2PP plate. The number of mutants emerging on the selective plates is recorded for 16 days. b Boxplots representing the distribution of new colonies appearing on each day in the 17 plates. (x), (-) and () represent the means, medians, and outliers, respectively. c Frequency of PCMs and rifampicin-resistant mutants. 109 bacteria (MG1655) were plated on TGP minimal medium supplemented with rifampicin (100 µg/ml) or on TG2PP plates. , PCMs; , RifR mutants. Each point represents the mean ± S.E.M. of 10 independent cultures. d Selection of PCMs in a mutS background. 109 MG1655 or ΔmutS::Cm bacteria were each separately plated on TG2PP plates. , PCMs from MG1655; , PCMs from mutS. Each point represents the mean ± S.E.M. of seven independent cultures

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