Fig. 2

Maximum likelihood phylogenetic trees of trypanosomatids. a Tree based on 18S rRNA gene sequences. b Tree based on 160 concatenated protein sequences. Numbers at nodes indicate posterior probability and bootstrap percentage, respectively. Values below 0.5 or 50% are replaced with dashes or omitted, absolute (1/100) supports are shown as black circles. The scale bar denotes number of substitutions per site. All clades representing described subfamilies or genera are collapsed in the 18S rRNA gene-based tree. The two species of biflagellate trypanosomatids studied here are highlighted; the sample S13-gut from the naturally infected Nemopoda nitidula is boxed