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Fig. 4 | BMC Biology

Fig. 4

From: Paxillin mediates ATP-induced activation of P2X7 receptor and NLRP3 inflammasome

Fig. 4

Paxillin promotes NLRP3 deubiquitination depending on extracellular ATP. a TPA-differentiated THP-1 macrophages were treated with ATP (5 mM) for 30, 60, and 120 min. b BMDM cells prepared from C57BL/6 mice bone marrow were treated with LPS (1 μg/ml) for 6 h, and the primed BMDM cells were stimulated by ATP (5 mM) for 15 and 30 min. c, d HEK293T cells (c) and Hela cells (d) were co-transfected with pFlag-NLRP3, pHA-Ubiquitin, or pMyc-Paxillin. e THP-1 cells stably expressing control lentivirus or Paxillin lentivirus were generated and differentiated into macrophages, which were then treated with ATP (5 mM) for 2 h. f THP-1 cells stably expressing shRNA targeting Paxillin were generated and differentiated into macrophages, which were then treated with ATP (5 mM) for 2 h. g BMDMs prepared from C57BL/6 mice bone marrow were infected by lentivirus that express shRNA targeting Paxillin for 3 days. Before stimulation, the BMDMs were treated with LPS (1 μg/ml) for 6 h, and the primed BMDMs were stimulated by ATP (5 mM) for 30 min

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