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Fig. 7 | BMC Biology

Fig. 7

From: Paxillin mediates ATP-induced activation of P2X7 receptor and NLRP3 inflammasome

Fig. 7

ATP induces Paxillin and NLRP3 localization near the plasma membrane. a Hela cells were transfected with pFlag-Paxillin and pHA-NLRP3, or co-transfected with pFlag-Paxillin and pHA-NLRP3, and treated with ATP (5 mM) for 2 h. Subcellular localization of Flag-Paxillin (green), HA-NLRP3 (red), and the nucleus marker DAPI (blue) was examined by confocal microscopy. b TPA-differentiated THP-1 macrophages were treated with ATP (5 mM) for 2 h. Subcellular localization of NLRP3 (green), the membrane marker Dil (red), and DAPI was examined by confocal microscopy. c TPA-differentiated THP-1 macrophages were treated with ATP (5 mM) for 2 h. Subcellular localization of NLRP3 (green), Paxillin(Y118) (red), DAPI (blue), and white light was examined by confocal microscopy. d TPA-differentiated THP-1 macrophages were treated with ATP (5 mM) for 2 h. Subcellular localization of Paxillin(Y118) (green), the membrane marker Dil (red), and DAPI (blue) was examined by confocal microscopy. e TPA-differentiated THP-1 macrophages were treated with ATP (5 mM) for 2 h. Subcellular localization of NLRP3 (green), Paxillin(Y118) (cyan), and the membrane marker Dil (red) was examined by confocal microscopy. f LPS-primed BMDMs were stimulated by ATP (5 mM) for 30 min. Subcellular localization of NLRP3 (green), the membrane marker Dil (red), and DAPI (blue) was examined by confocal microscopy. g LPS-primed BMDMs were stimulated by ATP (5 mM) for 30 min. Subcellular localization of Paxillin(Y118) (green), the membrane marker Dil (red), and DAPI (blue) was examined by confocal microscopy. h LPS-primed BMDMs were stimulated by ATP (5 mM) for 30 min. Subcellular localization of NLRP3 (green), Paxillin(Y118) (cyan), and the membrane marker Dil (red) was examined by confocal microscopy

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