Fig. 8
From: Paxillin mediates ATP-induced activation of P2X7 receptor and NLRP3 inflammasome
ATP induces Paxillin and NLRP3 membrane migration to facilitate the formation of the P2X7R-Paxillin-NLRP3 complex. a Subcellular fractionation of TPA-differentiated THP-1 macrophages in the presence of ATP (5 mM) for 30, 60, and 120 min. The protein levels of NLRP3, Paxillin, P2X7R, Caspase-3, and Calnexin in the cytosolic fraction (left) and membrane fraction (right) were determined by Western blot. b–e Membrane flotation assays of TPA-differentiated THP-1 macrophages post-nuclear lysates on a 10–45% Optiprep gradient under the treatment of ATP (5 mM) for 30 min in the presence or absence of 1% Triton X-100. The protein level of NLRP3, Paxillin, P2X7R, Caspase-3, and Calnexin was determined by Western blot in each 24 fractions and used as the input