Fig. 2
From: Crystal structure of the yeast heterodimeric ADAT2/3 deaminase
The in vitro and in vivo experiments to test the functions of ScADAT2/3. a The in vitro tRNA deamination assay coupled with cleavage by EndoV. The mutants are grouped according to their sources and biophysical properties and are shown above each panel. QuadruA: the ScADAT2-D124A/T125A/C126A/T127A mutant; TripleA1: ScADAT3-L212A/P213A/I214A; TripleA2: ScADAT3-D287A/Y289A/M291A. Note that the cleavage of the tRNAAla would generate two bands at 35 and 41 nt, respectively. b The knocked-out and complementation assays. Each mutant is diluted by 1×, 10×, and 100×, respectively, and 5-FOA is added (+ 5-FOA) or omitted (− 5-FOA) from the media