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Fig. 3 | BMC Biology

Fig. 3

From: miR-31-5p regulates cold acclimation of the wood-boring beetle Monochamus alternatus via ascaroside signaling

Fig. 3

The screening of targets of miR-31-5p. a The number of up- and downregulated genes after low-temperature acclimation. b KEGG enrichment of the differentially expressed transcripts (DETs) after low-temperature acclimation. The genes in the clusters with P < 0.01 were used to predict the putative targets by miRanda and RNAhybrid algorithm. c mRNA level of candidate targets of miR-31-5p by qPCR (n = 6). Student’s t test was performed to test significant differences, **P < 0.01; ***P < 0.01. d RNA immunoprecipitation (RIP) was performed with an anti-AGO-1 antibody; normal rabbit IgG was used as a negative control. qPCR analysis was performed to quantify the relative expression level of the target genes from the immunoprecipitates treated with agomir-31-5p (Ago-31-5p) and agomir-control (Ago-control) (n = 6). Student’s t test was performed to test significant differences, **P < 0.01. Gene legends: Amt, aminomethyltransferase; hairy, hairy; Suc, succinate-CoA ligase; α-actinin, alpha actinin; Acox1, acyl-CoA oxidase; Pdh, proline dehydrogenase. AC, low-temperature acclimation (4 °C); Control (25 °C). The last larval beetles used in RNA-seq (a, b) were collected at 5 Oct. 2016, qPCR validation (c), and RIP (d) at 10 Oct. 2017

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