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Fig. 2 | BMC Biology

Fig. 2

From: A Cas9-mediated adenosine transient reporter enables enrichment of ABE-targeted cells

Fig. 2

Identification and enrichment of base-edited cell populations using XMAS-TREE. a Schematic for identification and enrichment of adenosine base edited cells using XMAS-TREE. Cells are transfected with pEF-XMAS, pCMV-ABEmax, and pTS-sgRNA vectors. Post-transfection, flow cytometry is used to sort cell populations into reporter-positive and reporter-negative populations based upon mCherry and GFP expression levels. b Quantification of base-editing efficiency at various genomic loci in unsorted (white bar), mCherry-negative/GFP-negative (gray bar), mCherry-positive/GFP-negative (red bar), and mCherry/GFP double-positive (orange bar) isolated cells using XMAS-TREE-based methods. One-way ANOVA with Tukey post hoc test was performed. Statistical significance is only shown for the comparisons made between cells positive for the transfection reporter but not the editing reporter (i.e., mCherry-positive/GFP-negative) and cells positive for both (i.e., mCherry/GFP double-positive); *p < 0.05, **p < 0.01, ***p < 0.001. n = 3

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