Fig. 4

XMAS-TREE can be employed for the highly efficient base editing of human pluripotent stem cells (hPSCs). a Flow cytometry and b fluorescence microscopy analysis of hPSCs at various time points after transfection with pEF-XMAS-1xStop (top panels) or pEF-XMAS-2xStop (bottom panels), pEF-ABEmax, and sg(XMAS). c Quantification of base-editing efficiency at various genomic loci in unsorted (white bar), mCherry-negative/GFP-negative (gray bar), mCherry-positive/GFP-negative (red bar), and mCherry/GFP double-positive (orange bar) isolated hPSCs using XMAS-TREE-based methods. One-way ANOVA with Tukey post hoc test was performed. Statistical significance is only shown for the comparisons made between cells positive for the transfection reporter but not the editing reporter (i.e., mCherry-positive/GFP-negative) and cells positive for both (i.e., mCherry/GFP double positive); *p < 0.05, **p < 0.01, ***p < 0.001. n = 3