Fig. 5
From: A Cas9-mediated adenosine transient reporter enables enrichment of ABE-targeted cells
Highly efficient generation of clonal isogenic hPSC lines using XMAS-TREE. a Schematic for enrichment of adenosine base-edited cells using XMAS-TREE and reporter of transfection (RoT)-based approaches. b Quantification of relative base editing in mCherry-positive cells isolated using RoT and mCherry/GFP double-positive cells isolated using XMAS-TREE. Student’s t test; *p < 0.05, **p < 0.01, ***p < 0.001. n = 3. c Analysis of clonal editing efficiency in hPSCs that were targeted at the Site-3 locus using XMAS-TREE- or RoT-based methods