Fig. 6

Simultaneous adenosine base editing of multiple target sites in hPSCs using XMAS-TREE. a HPSCs were co-transfected with pEF-XMAS, pEF-ABEmax, and a pMT-sgRNA that simultaneously targeted Site-5/HBG1/HBG2. Flow cytometry was used to sort reporter-positive and reporter-negative cell populations and base-editing was quantified at target loci. One-way ANOVA with Tukey post hoc test was performed. Statistical significance is only shown for the comparisons made between cells positive for the transfection reporter but not the editing reporter (i.e., mCherry-positive/GFP-negative) and cells positive for both (i.e., mCherry/GFP double positive); *p < 0.05, **p < 0.01, ***p < 0.001. n = 3. b Quantification of relative base editing in mCherry-positive and mCherry/GFP double-positive cells isolated using RoT and XMAS-TREE, respectively. Student’s t test; *p < 0.05, **p < 0.01, ***p < 0.001. n = 3