Fig. 4
From: Lethal (2) giant discs (Lgd)/CC2D1 is required for the full activity of the ESCRT machinery
Reduction of shrub function causes a phenotype that resembles that of lgd mutants. (A) Expression of Gbe+Su(H) in a control wild-type disc. (B–C’) Depletion of shrub function by expression of UAS shrub-RNAi with hhGal4 tubGal80ts for 32 h (in the posterior compartment). The expression of Gbe+Su(H) is significantly increased in the depleted posterior area (B, C). The depleted cells accumulate Notch in enlarged endosomes (B’), but the expression of E-cad is not affected (B”). (C, C’) Z-stack of the disc shown in (B–B”) to show the apical-basal axis of the disc cells. The comparison of the cells of the anterior with cells of the posterior compartment reveals that the subcellular localisation of E-cad is not affected by the depletion of shrub. (D–H) Rescue of shrub null mutants by BACshrub-CHMP4B. (D) Expression of Gbe+Su(H) in a control wild-type disc. (E) A shrub mutant disc completely rescued by one copy of the control BACshrub-cDNA construct. (F) A similar rescue is observed with two copies of BACshrub-CHMP4B. (G) One copy of BACshrub-CHMP4B leads only to a partial rescue. Gbe+Su(H) is slightly ectopically expressed. It resembles the expression of Gbe+Su(H) in a hypomorphic situation of lgd shown in (H). Scale bars (A–B”) 50 μm; (D–H) 200 μm. At least ten wing imaginal discs were analysed for each genotype