Fig. 8From: Lethal (2) giant discs (Lgd)/CC2D1 is required for the full activity of the ESCRT machineryLgd cycles between the cytosol and the LM of the ME. (A’–C”) Depletion of Rab7 in the posterior compartment of a wing disc using an UAS rab7-RNAi construct continuously driven by hhGal4. (A’–C’) Magnification of yellow outlined area, located in the wild-type anterior compartment and (A”–C”) of the white box, located in the posterior Rab7-depleted compartment of the same wing discs shown in (A–C). The arrows point to Notch-positive MEs that are associated with Lgd-RFP. (D–F’) RNAi mediated depletion of vps4 function in the posterior compartment of the wing imaginal disc. hhGal4 tubGal80ts was used to drive expression of UAS vps4-RNAi for 24 h. The depletion results in an enlargement of Notch-positive MEs. Magnification of the region boxed in (D–F) is shown in (D’–F’). (G–I’) Depletion of shrub-RNAi for 38 h using enGal4 tubGal80ts leads to localisation of Lgd at enlarged ME. Magnification of the area boxed in (G–I) is shown in (G’–I’). (J–L’) Expression of the dominant-negative Shrub-GFP with hhGal4 for 15 h. Lgd-HA accumulates on the enlarged MEs together with Shrub-GFP. Magnification of the region boxed in (J–L) is shown in (J’–L’). Scale bars (A–L) 10 μm. At least ten wing imaginal discs were analysed for each genotypeBack to article page