Skip to main content
Fig. 8 | BMC Biology

Fig. 8

From: Lethal (2) giant discs (Lgd)/CC2D1 is required for the full activity of the ESCRT machinery

Fig. 8

Lgd cycles between the cytosol and the LM of the ME. (A’C”) Depletion of Rab7 in the posterior compartment of a wing disc using an UAS rab7-RNAi construct continuously driven by hhGal4. (A’C’) Magnification of yellow outlined area, located in the wild-type anterior compartment and (A”C”) of the white box, located in the posterior Rab7-depleted compartment of the same wing discs shown in (AC). The arrows point to Notch-positive MEs that are associated with Lgd-RFP. (DF’) RNAi mediated depletion of vps4 function in the posterior compartment of the wing imaginal disc. hhGal4 tubGal80ts was used to drive expression of UAS vps4-RNAi for 24 h. The depletion results in an enlargement of Notch-positive MEs. Magnification of the region boxed in (DF) is shown in (D’F’). (GI’) Depletion of shrub-RNAi for 38 h using enGal4 tubGal80ts leads to localisation of Lgd at enlarged ME. Magnification of the area boxed in (GI) is shown in (G’I’). (JL’) Expression of the dominant-negative Shrub-GFP with hhGal4 for 15 h. Lgd-HA accumulates on the enlarged MEs together with Shrub-GFP. Magnification of the region boxed in (JL) is shown in (J’L’). Scale bars (AL) 10 μm. At least ten wing imaginal discs were analysed for each genotype

Back to article page