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Fig. 6 | BMC Biology

Fig. 6

From: Feedback inhibition of AMT1 NH4+-transporters mediated by CIPK15 kinase

Fig. 6

NH4+ content and transport in cipk15 mutants. Plant seedlings were germinated and grown for 7 days in half-strength MS medium with 5 mM KNO3 as the sole nitrogen source, then all seedlings were starved for 2 days in half-strength MS medium without nitrogen. For NH4+ content analyses, a seedlings were collected after being starved for 2 days (−N), or after with 1 mM NH4Cl (NH4+), and 1 mM KNO3 (NO3−) for 1 h. For 15N-labeled uptake, b seedlings were collected after being starved for 2 days (−N) (1 mM 15NH4Cl was used for 15 mins for 15N-labeling), or after treatment with 1 mM NH4Cl (NH4+), and 1 mM KNO3 (NO3−) for 1 h (1 mM 15NH4Cl was used for last 15 mins for N15-labeling for conditions of NH4+ and NO3−). Each data point represents different experiments, in which seedlings n > 15, total n > 60) in Col-0 and two cipk15 knockout mutants and presented as box and whiskers. Center lines show the medians; box limits indicate the 25th and 75th percentiles as determined by Prism software; whiskers extend 1.5 times the interquartile range from the 25th and 75th percentiles, outliers are represented by dots. p, significant change compared to before submergence (two-way ANOVA followed by Tukey’s post-test)

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