Fig. 2
From: Oxidative stress mediates thalidomide-induced pain by targeting peripheral TRPA1 and central TRPV4
Genetic deletion of TRPA1 and TRPV4 attenuates mechanical and cold hypersensitivity evoked by thalidomide. a, b Time-dependent mechanical allodynia following intraperitoneal (i.p.) injection of thalidomide (Thal, 50 mg/kg) or Veh in Trpa1+/+, Trpa1−/−, Trpv4+/+, and Trpv4−/− mice. c, d Mechanical allodynia in Trpa1+/+ and Trpa1−/−, Trpv4+/+ and Trpv4−/− mice at day 7 following Thal (50 mg/kg, i.p.) or Veh and after the administration of HC-067047 (HC06, 10 mg/kg, i.p.) or Veh in Trpa1−/− mice and HC-030031 (HC03, 100 mg/kg, i.p.) or Veh in Trpv4−/− mice. e, f Time-dependent cold allodynia following Thal (50 mg/kg, i.p.) or Veh in Trpa1+/+, Trpa1−/−, Trpv4+/+, or Trpv4−/− mice. BL, baseline. Data are mean ± SEM, n = 6 mice. *P < 0.05 vs. Trpa1+/+/Veh Thal or Trpv4+/+/Veh Thal or Trpa1+/+/Veh Thal/Veh HC06 or Trpv4+/+/Veh Thal/Veh HC03; §P < 0.05 vs. Trpa1+/+/Thal or Trpv4+/+/Thal or Trpa1+/+/Thal/Veh HC06 or Trpv4+/+/Thal/Veh HC03; #P < 0.05 vs. Trpa1−/−/Thal/Veh HC06 or Trpv4−/−/Thal/Veh HC03. Two-way ANOVA followed by Bonferroni’s post hoc test