Fig. 5

Gene expression at late stages of regeneration. a Sagittal schematic of regenerating arm at late stages of regeneration with proximal (Prox.) and distal (Dist.) schematics of cross sections. Purple, epidermis; grey, skeleton; yellow, ACC; blue, RWC; pink, RNC. b Whole mount ISH (proximal and distal position as indicated at the top of the columns), using antisense probes for transcription factors. c Whole mount ISH (proximal and distal position as indicated at the top of the columns), using probes for known differentiation genes. For b and c, probe name is indicated at the bottom of the proximal image. On the left of each pair of ISH pictures, there is a summary of expression (blue) in cross schematics for the proximal (Prox.) and distal (Dist.) arm. Summary schematics of expression are based on focal plane observations of multiple samples; however, only one focal plane is shown here. In the distal figures, dotted squares indicate the differentiated terminal structure (terminal ossicle and podium) and black lines indicate the proliferating area. The summary expression data in the schematic is taken from the proliferating area. Scale bars = 50 μm