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Fig. 1 | BMC Biology

Fig. 1

From: No detectable alloreactive transcriptional responses under standard sample preparation conditions during donor-multiplexed single-cell RNA sequencing of peripheral blood mononuclear cells

Fig. 1

Schematic overview of experimental design. PBMCs from 8 healthy HLA-mismatched donors (tubes on left) were barcoded with MULTI-seq LMOs (black double-helix hybridized to red DNA barcode) and BD single-cell multiplexing kit (SCMK) antibodies (black antibody conjugated to teal DNA barcode). Cells were then strategically pooled to directly assess whether mixing HLA-mismatched PBMCs during scRNA-seq causes alloreactivity

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BMC Biology

ISSN: 1741-7007

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