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Fig. 2 | BMC Biology

Fig. 2

From: No detectable alloreactive transcriptional responses under standard sample preparation conditions during donor-multiplexed single-cell RNA sequencing of peripheral blood mononuclear cells

Fig. 2

MULTI-seq and SCMK classifications largely match in silico genotyping, with lower SCMK classification efficiency and bias against activated CD4+ T cells. a Sample classification results from three demultiplexing pipelines (e.g., deMULTIplex, souporcell, and demuxEM) projected onto MULTI-seq (top) and SCMK (bottom) sample barcode space. n = 4032 cells from microfluidic lane #3. b Classification frequencies across all PBMC cell types following SCMK sample demultiplexing. c SCMK unclassified cells in CD4+ T cell gene expression space. n = 6879 CD4+ T cells

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